Although he asserts his faithfulness to the Vulgate, Alvar Gómez is positive about the high literary value of his work, contrary to previous. To determine whether the predicted genes were responsible for the biosynthesis of KK-1, we performed heterologous production of KK-1 in Aspergillus oryzae by introduction of the cluster genes into the genome of A. The remaining seven genes in the cluster, excluding the regulatory gene kkR , were simultaneously introduced into the strain of A. These genes form an approximately kb cluster, which includes nine open reading frames, containing a non-ribosomal peptide synthetase NRPS gene.
It consists of 10 amino acid residues, including five N -methylated amino acid Slim Down Artinya, and has potent antifungal activity. Recently, the genome-sequencing analysis of C.
These genes form an approximately kb cluster, which includes nine open reading frames, containing a non-ribosomal peptide synthetase NRPS gene.
To determine whether the predicted genes were responsible for the biosynthesis of KK-1, we performed heterologous production of KK-1 in Aspergillus oryzae by introduction of the cluster genes into the genome of A. The remaining seven Slim Down Artinya in the cluster, excluding the regulatory gene kkRwere simultaneously introduced into the strain of A. To evaluate the heterologous production of KK-1 in A. KK-1 was produced in variable quantities by a number of transformed strains, along with expression of the cluster genes.
The amount of KK-1 produced by the strain with the greatest expression of all genes was lower than that produced by the original producer, C. Therefore, expression of the cluster genes is necessary and sufficient for the heterologous production of KK-1 in A.
